For example, the sweet bulb or plantlet of Gladiolus can not grow normally after transplanting into the soil(best plastic nursery trays wholesale nz). For example, it can grow normally after 4 ~ 6 weeks of pre-treatment at low temperature of 2 ℃, and Lily has a similar phenomenon(128 cell plug trays). It is best to use a new, high-pressure sterilization resistant, transparent polypropylene plastic bottle cap with a layer of film on the top.
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Shoot tip culture is widely used in the rapid propagation of flower waste, such as chrysanthemum, rose, orchid, lily, carnation and so on(32 cell seed tray). means that the plants obtained by culturing the shoot tips (or lateral buds, drum buds) are all diploid and have stable genetic traits; leaves are autotrophic organs for photosynthesis of plants, and they are also the reproductive organs of some plants(best plastic nursery trays wholesale nz).
Flowers (including flower buds, ovules, ovaries, floral touches, pollen, anthers, petals, etc(162 cell plug tray).) The somatic tissues of many plant flowers have high vegetative reproduction capacity, and these tissues are close to rejuvenating sex cells(best plastic nursery trays wholesale nz). The mature embryos of embryo-flowered plants can normally germinate and grow on a simple medium. immature embryos are more difficult to culture in vitro(32 cell seedling tray).
At the same time, with the increase of maintenance time, the initial plant regeneration ability of callus may gradually decline until it is finally lost(105 cell plug flats). The explants of the same plant from different organs and tissues also have great differences in morphogenesis(128 cell seed trays). Organ culture is the main way of rapid propagation of flower abandoned plants(best plastic nursery trays wholesale nz). The shoot tip is the most active growth area, and it is easy to grow seedlings in vitro.
Among the various horticultural varieties of flowering plants such as narcissus, Dongcao, Begonia and rose, it is also found that the response in culture is different(21 cell plug trays). Generally speaking, in micropropagation work, woody plants and tall herbs should adopt stem segment culture, because in the culture medium, the stem segment is easy to sprout lateral buds and become materials for rapid propagation(best plastic nursery trays wholesale nz).
The most common applications are stem loss culture and stem segment culture(200 cell plug trays), because when the root, stem, leaf and other organs are cultured in vitro, except the ventral bud on the basal segment of the basal tip can continue to grow, other organs usually dedifferentiate first and form callus(best plastic nursery trays wholesale nz), The injured tissue was re differentiated to further form a complete regenerated plant(288 cell plug tray). In addition, there is another important reason.
In theory, any organ of a plant can be used as an explant for culture, but the difficulty of successful culture of different organs is different, and the mode of cell genesis and development is also different(98 cell seed tray). If virus-free culture is not required, the volume of stem tip should be larger, and the survival rate is in direct proportion to the size of stem tip(best plastic nursery trays wholesale nz). The genetic characters of regenerated plants obtained from callus culture are often unstable.
This is because the virus exists least in the meristem of the stem tip(15 cell seed trays). The latter two situations mainly occur on immature embryos, and generally require comprehensive nutrients and suitable growth regulators(105 cell seed starting trays). The selection period of explants should usually be selected when the tissues or organs of the explants are in the most vigorous growth period, and their cell division and regeneration capabilities are the strongest(best plastic nursery trays wholesale nz).
Under normal circumstances, juvenile tissues have a higher morphogenetic ability than old tissues(best plastic nursery trays wholesale nz). The flower plants cultivated in vitro using leaf tissue and successfully have been anthurium, staghorn fern, chrysanthemum, gladiolus, tiger orchid, fuchsia, flower Leaf taro and so on(72 cell propagation tray). Some herbs, especially those short and lack obvious stems, can use leaves, petioles, pedicels, petals, filaments and anthers as explants.
Stem segment culture is widely used in the rapid propagation of flower abandoned plants, such as rose, chrysanthemum, peony, big rock system, Michelia, etc(best plastic nursery trays wholesale nz). Bulbs were cultivated with tulips, Narcissus, lily, hyacinth and so on. Tulip, vanilla, Clivia, gladiolus, etc. are cultivated by flower stems(200 cell seed trays). Once the above-ground stems are cut off, the crypto buds will be released from the suppression state and cluster buds will germinate.
According to reports, the floral plants that use root in vitro culture to obtain regenerated plants include kumquat, geranium, fired flower, clivia, kidney cane and so on(72 cell plug trays). Because the leaf tissues of many flower plants have strong regenerative ability, they can be directly induced to form buds, roots and other organs when cultured in vitro(51 cell plug trays); Leaves, roots(best plastic nursery trays wholesale nz). Explants should be selected according to the purpose of culture.
Usually, cell proliferation may occur on the culture medium to form traumatic tissue, and then re-differentiate to form multiple embryoid bodies or bud primordia(best plastic nursery trays wholesale nz). such as Clivia, marigold, bunch of red, petunia, lily, etc(seedling tray 104 holes). Therefore, embryo culture generally has three methods: rapid germination and seedling formation on the medium; second, continued "embryogenic growth" on the medium, and finally the formation of plantlets.
The organs and tissues of stems, bulbs, and flower stems are not as good as the base tip, but the source of materials is abundant(162 cell seed starting trays). If the medium is selected appropriately, and the type and concentration of growth regulating substances are also appropriate, good results can also be obtained(50 cell plug trays). The root neck may contain suppressed crypto buds(best plastic nursery trays wholesale nz). When using leaves as explants, the effect of using young leaves is better.
In addition, the stem disorder is used for virus-free culture, which can improve the quality and yield of various infected flowers and play the role of purification and rejuvenation(50 cell propagation trays). For example, the ability of in vitro culture of Duteng flower stems to produce roots weakens with the increase of stems. Clivia, chrysanthemum, phlox, gladiolus, etc. have been successfully cultivated in vitro using various tissues of flowers as explants(best plastic nursery trays wholesale nz).