Blind water management or experience management led to poor growth or serious diseases of vegetables in the durian room(black plastic plant pots wholesale nz). It is impossible to create appropriate water environment according to the water requirements of greenhouse vegetables at the growth stage(1 gallon nursery pots wholesale). The appropriate medium hardness is that the medium is just solidified and there is a small amount of water on the surface.
Black Plastic Plant Pots Wholesale NZ MOQ:1000pcs! 19 Years Experience Black Plastic Plant Pots Supplier, 35,000m² Workshop Area, Serving 3,000+ Customers!
Before use, add a certain amount of cold water to the bottom of the pot, put the sub packed culture medium into the autoclave, cover it tightly, and place the pot on the electric furnace for heating(black plastic plant pots wholesale nz). When the pressure in the pot rises to 0.5kgcm, open the vent valve, discharge the cold air in the pot, and then close the vent valve(72 cell propagation trays wholesale). The key to preparing a medium with appropriate hardness is the appropriate amount of agar.
Otherwise, the temperature in the pot is inconsistent, the cold air is compressed to the bottom of the pot, and the temperature of the bottom layer is less than 122 ℃, so that the culture medium at the bottom layer cannot be fully sterilized(black plastic plant pots wholesale nz). Due to poor ventilation, they often rot and die; when the medium is too hard, Prevent the division and expansion of explant cells(3 gallon nursery pots wholesale). Soil pH value has direct and indirect effects on crops.
The pore size of the bacterial filter membrane is less than or equal to 0.45um(black plastic plant pots wholesale nz). The principle of filtration sterilization is that after the solution passes through the filter membrane, the bacterial cells and spores are blocked because they are larger than the pore size of the filter membrane. When preparing a solid medium, two extreme phenomena often occur(1.5 gallon plant pot): the medium does not solidify or the medium is too hard.
In addition, the adsorption force of the filter membrane often makes the bacteria smaller than the pore size of the filter membrane unable to pass through(black plastic plant pots wholesale nz). When heating sterilization, pay attention to keep the pressure and temperature constant(200 cell plug trays supplier). Low temperature leads to insufficient sterilization, and high temperature is easy to invalidate some components of the culture medium(5 gallon nursery pots wholesale). The pH value is not affected by agar.
Large scale factory production requires the purchase of high-capacity autoclaves(black plastic plant pots wholesale nz). When the pointer of the pressure gauge approaches "0", open the vent valve to discharge the remaining steam, and then open the pot cover to take out the culture medium. Sealing film can also be used to cover the bottle mouth(2 gallon nursery pots wholesale). Press the solution into the filter membrane, and the solution dripping from the needle tube part is a sterile solution.
High temperature and high pressure sterilization significantly changed the p value of MS medium(7 gallon nursery pots wholesale). When the content of barbiturate is high, the decrease range of pH value increases(black plastic plant pots wholesale nz). The vacuum filter device is mainly composed of a filter and an air extraction system(104 cell seed starting trays). Put the glassware and other articles to be sterilized into a high-pressure disinfection pot and sterilize them at 1.lkg cm pressure and 122 ℃ for 20 minutes.
The cotton stopper can prevent microbial pollution and free gas exchange(105 cell seed starting trays), but it is best to wrap a layer of aluminum box or kraft paper outside the cotton stopper to prevent moisture absorption during high-pressure sterilization and easy pollution in the future(black plastic plant pots wholesale nz). The direct impact is that too high or too low pH in the rhizosphere environment will damage the roots of crops, and crops have the most suitable pH range.
Glassware and metal utensils (except plastic products and blades that are not resistant to high temperature) can be sterilized with dry heat(black plastic plant pots wholesale nz). Before disinfection, wrap them with aluminum pins, put them into a drying oven and disinfect them at 160 ℃ for 4 hours, or use high temperature and high pressure disinfection(10 gallon nursery pots wholesale). After sterilization, cut off the power supply to slowly reduce the pressure in the high-pressure pot.
Because it is better to use a new type of high-automation medical autoclave, which is safe and accurate(105 cell seed starting trays). Before use, assemble several parts of the injection filter sterilizer according to the requirements of aseptic operation, insert the syringe that sucks the sterilized solution to be filtered into the socket of the bacteria filter and push the piston rod of the syringe(black plastic plant pots wholesale nz). Sterilizer 1 used shall be sterilized in advance(200 cell seed starting trays).
When the medium needs to be added with some drugs that cannot be sterilized by high temperature(15 gallon nursery pots wholesale), the medium without these substances should be sterilized by high temperature, then placed on the ultra-clean workbench to cool to 40 ℃, and then filter sterilized The solution is added to the culture medium in sequence according to the planned dosage, and it can be used after shaking and solidifying(black plastic plant pots wholesale nz).
Due to the large difference in the quality of commercially available agar, it takes several experiments to determine its dosage(25 gallon nursery pots wholesale). In addition, the heating and sterilization time should not be too long, otherwise the medium will not be easy to solidify and cause some components of the medium to become invalid(black plastic plant pots wholesale nz). The powder and shaking medium can be scattered, and the explants are easy to insert and not fall during inoculation.
Pollution sources may include: culture container(20 gallon nursery pots wholesale); culture medium itself; explants; inoculation equipment; inoculation room environment; culture room environment; inoculation personnel's operation errors, and endophytic bacteria contamination. is that the high temperature and high pressure sterilization time of the medium is too long(72 cell seedling trays wholesale), which denatures the agar and does not solidify the medium(black plastic plant pots wholesale nz).
For the sake of caution, the sterilized filtrate can be placed at room temperature for 2 to 3 days, and no bacteria can grow before use(black plastic plant pots wholesale nz). When the medium is not solidified or is too soft, the explants are poured or submerged in the medium because they cannot stand upright(15 cell propagation trays wholesale). The filter holding liquid flows from the funnel through the filter membrane to the suction bottle with a vacuum pump or an electric attractor.
Filter sterilization is sometimes because the filter is not tight or difficult to control, and often fails to achieve thorough sterilization(128 cell seed trays wholesale). At the same time, it hinders the conduction and diffusion of nutrient elements and growth regulators in the medium, which is not conducive to the absorption of explants, making it difficult for cells to divide and organs to occur(black plastic plant pots wholesale nz). This is directly related to the success of the tissue culture(128 cell seedling trays wholesale).
When adjusting the pH value of the culture medium, the growth habit of the culture material should be considered(21 cell propagation trays wholesale). In addition, the reasons for the non-solidification of the medium are as follows: the pH of the medium is inappropriate, too much acid or too much alkali will cause the failure of the medium preparation, the suitable pH range is 5.2 ~ 6.2, and precision can be used(black plastic plant pots wholesale nz). pH test paper or acidity meter measurement.