The culture medium can be autoclaved for 20 minutes under a pressure of 3214.44Pa(plant trays without holes). The matrix should have more moisture within one week after the insertion, which will help the formation of the incision callus; in the future, reduce the moisture appropriately to prevent the base from rot(5 inch succulent pot). The management principle after support is to ensure that the best environmental conditions for rooting of cuttings are maintained.
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The environment around the cuttings is required to maintain an air mixture of about 90% to prevent excessive wilting of the cuttings; different plant species have different habits, and the required environmental humidity conditions are very different(v9 nursery pots). But not trapped in the medium. Immediately spray water (mist) with fine-eyed water (mist) and add a glass or plastic cover(7 inch plastic flower pots). You can remove the cover and irrigate with a nutrient solution with halved nutrients.(cheapest plastic flower pots wholesale direct belgium)
Disinfection of plant materials, tender stems, receptacles, petioles, leaves, young flower buds, etc(3.5 inch square plastic pots). Remove the excess part, then rinse, wash and rinse with detergent, put it on the super clean table in the inoculation room, soak in 70% alcohol for half a minute to disinfect the surface, then disinfect in 10% bleach solution for about 10 minutes(11 inch plastic plant pots), take it out Then rinse with sterile water for 4 to 5 times to inoculate (see appendix 2). After 4 weeks, it can be used for routine cultivation management.
(cheapest plastic flower pots wholesale direct belgium)After connecting, seal the bottle. It is the basic method of bulbous flower reproduction(10 inch nursery pots). The ability of different kinds of bulbous plants to develop meristem and regeneration bulb varies with the type (variety). It is suitable for most perennial flower holders. The garden soil contains organically rich, high-quality loose garden soil, which can be used as an open seedbed support substrate after being exposed to sunlight(plastic growing containers). Finally, add distilled water to set it to IL.
is usually cultured under constant temperature and light conditions(4 gallon plant pot). After a period of time, the culture material is transferred from the medium for inducing callus to the differentiation medium or directly to the rooting medium after a period of time. It varies according to the type of culture; the temperature is generally around 23~26℃(1 gallon plastic flower pots); the light has an effect on organ formation, the general range is 1000~3000Lx, 12~16 hours a day, higher than 3000Lx has a strong inhibitory effect.
After the test tube seedlings take root, take them out of the bottle in time, wash away the remaining medium, and transplant them into the prepared substrate(round plastic flower pots). MS medium is the most commonly used medium in rapid propagation, in which inorganic salts and organic active substances are relatively complete, which is suitable for in vitro culture of many plants(polypropylene plant pots). Calcium chloride should be added last to avoid precipitation such as calcium phosphate.(cheapest plastic flower pots wholesale direct belgium)
The method used to prepare ILMS culture medium is now introduced as follows(9cm square plant pots): The large elements can be prepared into a 10-fold concentrated mother liquor, that is, the dosage of various reagents is increased by 10 times, and they are dissolved separately. Watering is not required for 34 days, and watering later With a small amount of water, the leaves are straight after 1 week(large black plastic flower pots). Different hormones and concentrations are selected according to the types and organs of cultivated plants.