The general rule of the degree of difficulty in rooting is: woody plants are harder than herbs, adult trees are harder than young trees, and trees are harder than shrubs(plastic plant pots wholesale suppliers bulgaria). If the test tube seedlings are transferred blindly without mastering the relevant theories and technologies, it is bound to cause high mortality and waste all previous efforts(v9 plastic pots).
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The influence of light intensity and photoperiod of culture conditions on root induction is inconsistent(10cm plastic plant pots). The situation is more complicated, please refer to relevant information, but the test tube seedling rooting requires a certain suitable temperature, generally 16~25℃, too high or too low are not conducive to rooting, and the optimal rooting temperature is different for different plants(plastic plant pots wholesale suppliers bulgaria).
For example, when the small bulbs of gladiolus obtained on MS primary medium are transplanted on fresh MS secondary medium, other materials and some plants need weak light or dark conditions for rooting(32 cell seedling tray). Activated carbon can play a role in shading, and can also absorb some harmful substances that inhibit growth(plastic plant pots wholesale suppliers bulgaria). Therefore, activated carbon is often added to some rooting media at a dosage of about 0.5%.
In addition, the medium H value also has a certain relationship with the rooting of test-tube plantlets(162 cell plug tray). Generally, the pH value suitable for the rooting of flower test-tube plantlets is between 5.0 and 7.0. In addition, it is convenient for transplanting in different places or long-distance transportation(200 cell trays). Take out 2 ~ 3cm long rootless test tube plantlets from the bottle by pot insertion method. There are few or no root hairs(plastic plant pots wholesale suppliers bulgaria).
At the same time(50 cell plug trays), for some varieties that are difficult to root in the test tube or in order to reduce the production process, the method of rooting outside the test tube can be used, which can save the rooting medium, simplify the cultivation procedure, and shorten the seedling time and reduce the production cost(plastic plant pots wholesale suppliers bulgaria). This method has fast cutting speed(40 cell seed tray inserts), simple operation, high survival rate, and shortens the production cycle.
The growth environment of test tube seedlings is generally weak light, constant temperature, high humidity, and heterotrophic(plastic plant pots wholesale suppliers bulgaria). The following methods are often used for rooting outside the test tube(200 cell plug trays): after the root primordium is formed in the test tube, the root system of the transplanted test tube plantlet is prone to mechanical damage when transplanting out of the bottle, which affects the survival rate.
Within 1 to 2 weeks of the initial stage of transfer, cover with plastic film, keep the relative humidity about 85%, and shade about 50%(72 cell plug trays). The temperature depends on the plant species, generally kept at about 22-28°C(plastic plant pots wholesale suppliers bulgaria). When the root system is formed, the seedlings change from heterotrophic to autotrophic, and when they can absorb water by themselves, the environmental humidity can be appropriately reduced.
"If you use a high-salt medium with a concentration of 12, 13, or 14 or a medium with a lower total salt concentration(32 cell tray), it is conducive to root differentiation(plastic plant pots wholesale suppliers bulgaria). Take out the rootless test-tube plantlets with a length of 1~3cm from the bottle, treat the base with 50~100mg LABT No. 1 rooting powder solution for a few seconds or longer, and directly insert them into the greenhouse(32 cell plug tray). Or the seedbed in the greenhouse.
Or divide the protocorm into countless small pieces; or separate the embryoid bodies(seedling tray 104 holes); or cut the clustered shoots from the callus together with the callus block (the size of the bud is about 0.5~1.0cm), and then The divided small pieces of culture material are inoculated on fresh subculture medium for proliferation and culture(plastic plant pots wholesale suppliers bulgaria). One of the most direct reasons is that the transfer survival rate of tissue culture seedlings is low.
After the base is treated with 50 ~ 100mg labt No. 1 rooting powder solution for several seconds, insert them into the matrix of the flowerpot, the insertion depth is about LM, add rooting nutrient solution into the matrix, 10 ~ 20 rootless plantlets can be inserted into each pot, cover the flowerpot with material film to maintain high humidity(50 deep cell plug trays), Place in the greenhouse where there is diffuse light or shade(plastic plant pots wholesale suppliers bulgaria).
When the temperature is controlled at 22 ~ 28 ℃, new roots with autotrophic ability can be produced after 3 ~ 4 weeks of culture(v13 plastic pots). Then gradually switch to normal seedling management(plastic plant pots wholesale suppliers bulgaria). Whether the test tube plantlets obtained from flower discarding tissue culture can be widely used in production and whether they are effective depends on the last pass, that is(pp woven ground cover), the transfer of test tube plantlets in the fourth stage.
Most of the leaves with undeveloped cuticle and wax layer or without test tube plantlets differentiated and grew under high humidity, weak light and abnormal conditions lacked or even did not have leaf surface protective tissue(mixture of peat soil and vermiculite 1:1)(72 cell plug flats). After the bottle is out, the growth environment of transplanting has changed greatly, and has changed from heterotrophic to autotrophic(plastic plant pots wholesale suppliers bulgaria).
These changes are very easy to cause the test tube seedlings to wither and die due to water loss(105 cell seed starting trays). Basic medium the basic medium used to induce rooting, in addition to using the same medium as the first stage, often needs to reduce the concentration of inorganic salt(128 cell trays). When the root system of test tube plantlets has not yet formed, but only the root primordium, transplanting is conducive to improve the survival rate(plastic plant pots wholesale suppliers bulgaria).
The reasons are analyzed from the aspects of morphological anatomy and physiological function as follows(v14 plastic pots): some rootless plants, especially woody plants, can continue to grow and proliferate in Tube Propagation, but do not take root or the rooting half is very low and can not be transplanted(plastic plant pots wholesale suppliers bulgaria). For example, peony tube propagation can not be used for mass production because the rooting problem has not been well solved.
The root of test tube seedlings of some flowers is lack of root hairs or even no root hairs(128 cell seed trays), which leads to the extremely low absorption capacity of the root system, and the trace water absorbed by the root system is difficult to meet the consumption of seedling transpiration(10.5 cm plant pots), The water in the seedling is difficult to reach balance, so it withers and dies(plastic plant pots wholesale suppliers bulgaria). Then, the transplanting process of test tube seedlings is complex.
Ellen et al. (1974) observed the fine structure of leaf epidermal wax of test tube plantlets cultured at the shoot tip of Carnation with scanning electron microscope and found that 96% ~ 98% of the test tube plantlets had smooth and unstructured epidermal wax layer(105 cell seedling trays), Or only a few rod-shaped wax particles(plastic plant pots wholesale suppliers bulgaria). The addition of activated carbon is beneficial to promote the rooting of some plant test-tube seedlings.