As a carbon source, sugar provides a carbon skeleton for cells to synthesize new compounds, and provides substrates and energy for cells' respiratory metabolism(high quality plastic nursery pots wholesale georgia). Sugar can also maintain a certain osmotic pressure (1.5 ~ 4.impa). The commonly used carbon source is vegetable sugar, with a concentration of 2% ~ 5%(8 inch nursery pots). The basic medium ensures the survival and minimum physiological activities of the culture.
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Glucose and fructose are also good carbon sources, which can make many tissues grow well(3 gallon nursery pots). When sucrose was used as carbon source, the roots of dicotyledons grew best, while when glucose was used as carbon source, the roots of monocotyledons grew best(11 inch plastic plant pots). It has the effect of helping active substances play a role, can promote the growth of callus, and can also promote the formation of compacts and buds(high quality plastic nursery pots wholesale georgia).
In terms of drug activity(7 gallon nursery pots), 2.4-D has the strongest effect, NAA and IBA are in the middle, and LAA is the weakest(high quality plastic nursery pots wholesale georgia). In order to reduce the production cost, commercial white granulated sugar or even non decolorized brown sugar is used to replace chemically pure or analytically pure sucrose in plant rapid colonization in many areas(large plastic planters). In anther culture, sucrose concentration is often increased to promote differentiation.
Inositol can often be converted from glucose phosphate and further form pectin(plastic plant pots wholesale). Therefore, it can sometimes be omitted in rapid propagation. When adenine and its sulfate were added to the culture medium, they could promote the formation and growth of buds(nursery pots canada). The general dosage of inositol is 50 ~ 100mgl(high quality plastic nursery pots wholesale georgia). Adding a small amount of inositol 100mgl to the culture medium can promote the effect of vitamin B.
For the rapid growth and differentiation of culture materials, in addition to various vitamins, one or more amino acids are often added, including glycine, arginine, glutamic acid, glutamic acid, aspartic acid and alanine(6 inch nursery pots). After dissolution, add Yanguan water to the required amount(high quality plastic nursery pots wholesale georgia). The results show that high-pressure sterilization can hydrolyze sucrose into fructose and glucose which can be used more effectively(10 inch nursery pots).
Generally, growth stimulating hormones are dissolved in 95% alcohol or 0(high quality plastic nursery pots wholesale georgia). IN hydrochloric acid solution, and are often stored in the refrigerator for later use(grow bags wholesale). They can affect plant cell division, differentiation and development in a very small amount, and affect physiological and biochemical activities such as plant morphogenesis, flowering, fruiting, maturity, abscission, aging, dormancy, germination and so on(custom plant pot).
It should be noted that gibberellin does not attach to heat, and most of it becomes invalid after autoclaving, so it is best to use 95% alcohol to prepare the mother liquor and store it in the refrigerator for each use(hanging baskets wholesale). Only the addition of appropriate plant hormones can induce the expected changes such as cell division, differentiation, callus growth, bud and root differentiation or embryoid development(high quality plastic nursery pots wholesale georgia).
In tissue culture, commonly used synthetic analogues are(high quality plastic nursery pots wholesale georgia): throat acetic acid (AA), grin butyric acid (IBA), tea acetic acid (NAA), dichlorophenoxyacetic acid (2.4-D), gibberellin ( GA) etc(plastic bonsai pots). In the process of inducing certain physiological changes and morphogenesis of culture, timely and appropriate addition of appropriate plant hormones is the most important factor to promote the occurrence and development of these changes(1 gallon nursery pots).
Natural growth stimulators are affected by the synthesis and decomposition of enzymes in the body, and are also susceptible to light oxidation(seedling trays wholesale). Various cytokinin molecules contain adenine. In the differentiation and growth medium for inducing buds, appropriate addition of adenine is beneficial to the growth of cytokinins(20 gallon nursery pots). Synthetic activity is conducive to plant tissues to coordinate their cell division and differentiation(high quality plastic nursery pots wholesale georgia).
Artificially synthesized analogues are called plant growth regulators(plug plant trays), and the various plant hormones referred to have broad meanings, including plant growth regulators(high quality plastic nursery pots wholesale georgia). Manganese or manganese affects cell elongation. Most plant cells can synthesize all necessary vitamins in culture, but the quantity is obviously insufficient(5 gallon nursery pots). Small molecular compounds such as glycine and alanine can be directly absorbed by plant cells.
Generally speaking, 2.4-D is generally used for cell activation to promote embryoid body formation(plastic plant trays). NA, BA, AA, etc. are used to induce the differentiation stage, so that the cell clusters or tissues formed by dedifferentiation and in the age-assisted state will regenerate specialized cells with high-level tissue structures such as roots, buds, and embryoid bodies(high quality plastic nursery pots wholesale georgia). Adenine is one of the precursors for the synthesis of various cytokinins(2 gallon nursery pots).
Plant hormones produced in plant metabolism are natural compounds(high quality plastic nursery pots wholesale georgia). The main function of gibberellin is to break dormancy, promote the elongation of seedling stems, and affect the differentiation of cambium cells; in addition, it can also break the dormancy of seeds, tubers, bulbs, etc., and make them germinate in advance(weed pots). In tissue culture, erythrotoxin It can stimulate the normal development of adventitious embryos into plantlets.
Generally speaking, isolated tissues and organs are easy to Senescence quickly, adding cytokinin to the culture medium can delay its aging process(15 gallon nursery pots wholesale). Therefore, there are more than 10 kinds of natural cytokinins in nature, and there are also synthetic cytokinins(plastic plant pots nz). The more commonly used cytokinins are benzylaminopurine (BAP), caryladenine (6-BA), isopentenylaminopurine (2-iP) and kinetin (6-citrylaminopurine) and so on(high quality plastic nursery pots wholesale georgia).
The main function of major cytokinins is to promote cell division and expansion(4 inch plastic pots wholesale), make stems thicker, and inhibit stem elongation; guide bud differentiation and promote lateral bud germination; inhibit senescence(high quality plastic nursery pots wholesale georgia). Cytokinins are easily soluble in dilute hydrochloric acid (0.1~1N). In plant tissue culture work, commonly used plant hormones (plant growth regulators) include growth stimulators and cytokinins(10 gallon nursery pots).