The plant material initially inoculated is called explant(4 inch plastic plant pots wholesale price). Carry out the final sterilization treatment on the super clean workbench(4 inch nursery pots wholesale). Before operation, start the machine for more than 10 minutes, then place a clean beaker (the size depends on the amount of materials) on the super clean workbench, and then put the processed materials into the beaker.
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For various instruments used in aseptic operation, such as silver, solution knife, dissecting needle, scissors and open head shovel, the method of burning and sterilization is adopted(10 inch nursery pots). Expel bubbles and improve sterilization effect. Its toxic effect is not as fast as that of bleaching powder(4 inch plastic plant pots wholesale price). After storage for a period of time, the color will deepen, but its quality will not be affected(8 inch nursery pots), Integrated iron is best stored in the refrigerator.
Put the prepared sterilization solution and sterile water on the super clean table(15 gallon nursery pots), transfer the drained plant material from the beaker to the sterilized jar, watch the time, and immediately pour the sterilization solution, such as adding a few drops of Tween-80, then cover the bottle stopper and gently move the jar, so as to make all parts of the surface of the plant material fully contact with the natural bacteria solution(4 inch plastic plant pots wholesale price).
Generally(7 gallon nursery pots), sodium hypochlorite can decompose to produce chlorine with bactericidal effect and volatilize(4 inch plastic plant pots wholesale price); Hydrogen peroxide can decompose to produce oxygen with bactericidal effect to form harmless compounds; Mercuric chloride (shengrou) is highly toxic and has the best sterilization effect on the surface of plant materials(10 gallon nursery pots). However, it is difficult to remove the residual mercury after sterilization.
Then, it should be remembered that EDTA integrated iron is commonly used, which is prepared from ferrous sulfate (fes0.. 7ho) and sodium ethylenediamine tetraacetate(large plastic planters). Dissolve them respectively (heat them to fully dissolve them), and then pour the two solutions into another beaker at the same time for integration for about 30 minutes(4 inch plastic plant pots wholesale price). The color is light yellow(11 inch plastic plant pots). Generally, it will be shown after a period of culture.
When sterilizing, soak in 70% alcohol for a few seconds, and rinse with sterile water for 2~3 times(plant pots nz). Tianji is also toxic to plant tissues. Due to its strong penetration, the treatment time should be strictly controlled. It is prepared by adding L ~ 2 jiao spoons per 100ml of water(grow bags wholesale). Refer to the high-pressure sterilization to remove the culture medium and allow the culture medium to cool at room temperature(4 inch plastic plant pots wholesale price).
When the predetermined time is approaching, start to pour the sterilization solution into another prepared beaker, and be careful not to pour out the material(4 inch plastic plant pots wholesale price). According to the cleanliness of the fruits and seeds, rinse with tap water for a few minutes, and then rinse with 70% alcohol for 3 to 4 seconds(plastic plant trays). Since most of these materials are buried in the soil, the materials are often damaged and soiled, and disinfection is more difficult.
Different plant materials have different sensitivity to sterilizing agents(4 inch plastic plant pots wholesale price). Generally, pour sterile water immediately after pouring and shake gently(nursery pots canada). Sterile water flushing takes about 3 minutes each time, and the flushing times vary according to the type of sterilization solution used, ranging from 3 times to 10 times(6 inch nursery pots). For those that are difficult to sterilize, it can also be sterilized with a 0.1% mercury liter solution for 5 minutes.
Therefore, the type, concentration and treatment time of sterilizing agent and the times of washing with sterile water should be correctly selected to minimize the damage to plant materials(5 gallon nursery pots). For some small or floating plant materials, gauze and plastic screen window can be used to tie the cup mouth(4 inch plastic plant pots wholesale price). Soak and stir with washing powder. The concentration of washing powder solution can be appropriately higher.
The sterilization methods of different materials are introduced below(1 gallon nursery pots). Wetting agents include Tween-80 or Tween-20(4 inch plastic plant pots wholesale price). Add a few drops or use 0.1% concentration to help other bactericides to wet the entire tissue surface and enhance the sterilization effect(plastic bonsai pots). The anthers used for culture are generally immature, and they are usually in a sterile state because of the protection of the flower heads, petals or glumes on the outside.
According to the age, tenderness of the material and the firmness of the branches, soak in 2%~10% sodium hypochlorite solution for 10~15(plastic hanging baskets wholesale). Or soak in 0.1% mercury solution for 10-15 minutes, and then rinse with sterile water 4 times before inoculation. Before each vaccination, and then dried with absorbent paper, the ground should be cleaned and sanitary, and 70% of the sperm spray should be used to settle the dust in the air(4 inch plastic plant pots wholesale price).
So just sterilize the whole flower bud or juvenile(4 inch plastic plant pots wholesale price). Generally, it is soaked in 70% alcohol for several seconds, then rinsed with sterile water for 2 to 3 times, then soaked in the bleach solution for 10 minutes, and finally rinsed with sterile water for 2 to 3 times to inoculate(custom plant pot). I don’t know the moisture requirements of the vegetables themselves, and the suitable soil pH range for common vegetable crops.
After washing, it can be inoculated(plastic plant pots wholesale). For the fruit, as long as the surface is rinsed with sterile water for 2 to 3 times for 10 minutes with a 2% sodium hypohydride solution, the inner seeds or tissues can be cut for inoculation(seedling trays wholesale). For the seeds, it is more complicated(4 inch plastic plant pots wholesale price). First, soak them in a 10% calcium hypochlorite solution for 20-30 minutes, or even a few hours (depending on the hardness of the seed coat).
For seeds used for embryo or endosperm culture, sometimes the seed coat is too hard and difficult to dissect during inoculation(20 gallon nursery pots). The seed coat can also be removed in advance, and then inoculated with 4% to 8% sodium hypochlorite solution for 8 to 10 minutes, and rinsed with sterile water After that, the embryo or endosperm can be removed for inoculation(2 gallon nursery pots). generally can be rinsed with tap water first(4 inch plastic plant pots wholesale price).
Convex uneven areas and bud scales or bracts should be carefully scrubbed with a brush or brush to remove dirt(3 gallon nursery pots), cut off damaged and heavily contaminated parts with a knife, put in 70% alcohol solution Rinse the inside quickly, then disinfect with 0.1%～0.2% mercury liter solution for 5-10 minutes, or soak in 6%-8% sodium hypochlorite solution for 5-15 minutes, and then rinse with sterile water 4-6 times(4 inch plastic plant pots wholesale price).