Because the shoot tip meristem is not self-sufficient in auxin, auxin synthesis occurs only in the second pair of leaf primordia(plastic nursery plant pots wholesale usa). The main characteristics of virus are its simple structure and replication mechanism(v19 plastic pots). For some plants that are easy to transplant, the operation procedure can be simplified according to the specific and feasible alternative methods.
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In fact, shoot tip meristem with leaf primordia with a length of more than 500um can grow and develop in the medium without auxin, but the medium with auxin is better(plastic nursery plant pots wholesale usa). 6-benzylpurine (BA) is commonly used with a concentration of 0.5mgl(50 cell seed trays). The test tube seedling transfer technology introduced mainly aims at the plant species with great difficulty in transplanting, and the operation procedure is cumbersome.
Adding growth regulators to the culture medium plays an important role in stem growth and development, especially the type and concentration of growth regulators often play a decisive role(cheap plastic hanging baskets). The stems of this virus-free narcissus grow faster than the ordinary original species, have stronger viability, larger flowers than the infected plants, rich colors, and more flowers per stem(plastic nursery plant pots wholesale usa).
Many experiments show that in the early stage of culture(40 cell seed trays), a small amount of gibberellin (GA) is conducive to the survival and elongation of the stem tip, but if the concentration is too high or the use time is too long, it will have an adverse effect, so that the stem tip is not easy to turn green, the leaf primordium elongates rapidly(9cm plastic plant pots), but the growth point does not grow, and the whole stem tip finally turns brown and dies(plastic nursery plant pots wholesale usa).
It is emphasized that different kinds of flower discards have different responses to growth regulators, which must be flexibly mastered in combination with the culture situation(plastic nursery plant pots wholesale usa). Generally, the medium factors that play a role in shoot tip culture mainly include nutrients, growth regulators and physical conditions (solid or liquid, static or oscillation, etc.)(plastic ground cover for weed control).
Although the smaller the shoot tip in vitro, the better the effect of virus removal, the survival rate of the shoot tip is very low(36 cell seed trays). Generally speaking, MS medium is widely used in flower plant shoot tip culture(plastic nursery plant pots wholesale usa). Its high content of inorganic salt and organic matter can fully meet the needs of shoot tip growth and development. The shoot tip is not very sensitive to the physical state of the medium (liquid or solid).
In shoot tip culture, the hardness of the medium is also very important(v11 plastic pots). After 1 to 2 weeks of in vitro culture, the base tip did not increase significantly, but the color gradually turned green, and finally turned into small green spots. The shoot tip with leaf primordium has a higher survival rate(v23 plastic pots). Sometimes a small amount of callus is formed, and the stem tip is gradually elongated(plastic nursery plant pots wholesale usa).
When the shoot tip grows to LCM height (about 5 ~ 6 weeks of culture time), the light intensity can increase to 3000 ~ 4000lk(20 cell seed trays). Shoot tip culture is generally better at room temperature of 25 ℃ ± 2 ℃. Too high or too low temperature is unfavorable to the growth and development of shoot tip(plastic nursery plant pots wholesale usa). Otherwise, it is easy to form a mass of translucent callus and lose the ability to differentiate and grow.
At this time(v20 plastic pots), the shoot tip should be immediately transferred to a medium with a lower auxin concentration, or the culture temperature should be lowered, and the light intensity should be increased(plastic nursery plant pots wholesale usa). The tissue near the growth point should be as few as possible(nursery seedling tray). Generally speaking, the optimum light intensity at the beginning of shoot tip culture is 1000x, which increases to 2000x after 4 weeks.
The quality of the agar available on the market is different, and the amount is different(18 cell seed trays). The in vitro culture of some flowers, such as orchids, is very suitable for liquid culture medium, and the growth rate of differentiation is greatly accelerated(plastic nursery plant pots wholesale usa). Stone (1973) used a variety of narcissus to detoxify the shoot tip and obtained a clone free of Arabian mosaic virus and narcissus degenerative virus.
For the very small stem tip with only growth point(128 cell plug flats), in order to avoid rot and death caused by the stem tip sinking to the bottom in the liquid medium, it is best to build a filter paper bridge in the liquid medium and place the stem tip growth point on the paper bridge soaked in the medium(plastic nursery plant pots wholesale usa). However, it is also possible that the shoot tips cultured in vitro are in a dormant state due to the bud-harvesting season.
Under normal circumstances, the color of the shoot tip in vitro culture gradually turns green, and the base gradually becomes thicker(12 cell seed trays). Generally, there are 1 to 2 leaf primordia. This is also an important factor that can not be ignored(plastic nursery plant pots wholesale usa). Usually, the effect of top bud is better than that of side bud(12cm plastic plant pots). During the bud taking period, most flower abandoned plants pick buds in spring or early summer than in other seasons.
In addition, generally speaking, the greater the loss of the detached stem, the easier it is to survive, but the worse the effect of virus removal(plastic nursery plant pots wholesale usa). For most plants, this is caused by low auxin concentration or low cultivation temperature. It should be immediately transferred to a medium with a higher auxin concentration, or the culture temperature should be appropriately increased to promote the growth of the shoot tip(16.5cm plastic plant pots).
Within 1 week of inoculation, the stem tip became obviously thicker, and callus formed at the base(8 cell seed trays). The stem tip was not significantly elongated, and the color change was not obvious(plastic nursery plant pots wholesale usa). This is due to the high concentration of auxin in the medium(fabric grow bags wholesale). After about 4 weeks, you can see obviously elongated small stems, and the leaf primordium also forms visible lobules, which shows that each culture factor is very suitable.
The medium is more The softness is beneficial to the survival of the stem tip, and sometimes the amount of agar can be reduced to 4.0gL(plastic nursery plant pots wholesale usa). Or the light intensity is not enough, the temperature is too high, etc(112 cell seed trays). Factors such as general medium composition, explant size and culture conditions not only affect the regeneration ability of in vitro shoot tips, but also significantly affect the detoxification effect of shoot tips.
It was basically lighter all the time(plastic nursery plant pots wholesale usa). According to different types of shoot tip growth, timely and appropriate adjustment of the concentration of growth regulators (mainly auxin) and appropriate cultivation conditions can generally greatly increase the survival rate of shoot tips, sometimes reaching more than 80%(6 cell plant trays). Such a shoot tip can not only ensure a certain survival rate, but also eliminate most viruses.