Under suitable conditions, some use lateral buds to differentiate and enhance elegance, such as chrysanthemum, fuchsia, pine chrysanthemum, etc.(cheap 4 inch plastic nursery pots suppliers); some re-differentiate buds from around the leaves, such as insect cocoons, coleus, African violet, gloxinia, etc.(14 cm plastic plant pots); Yes The buds differentiated from scales or small stems, such as hyacinth, narcissus, lily, etc.
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This approach is also called bud proliferation in various countries(cheap 4 inch plastic nursery pots suppliers). The advantage is that the variation of genetic traits is small and the reproduction speed is fast(9cm flower pots). In vitro culture of plant leaves, petioles, stems, roots and floral organs are used to induce callus, and then the callus is induced to differentiate into adventitious buds and roots to form regenerated plants(72 cell seedling trays), such as hibiscus, longevity flower and five-star flower.
After being sterilized by the surface, insert the medium shallowly under aseptic conditions, the seedlings can germinate after a certain period of time, and then cut into single bud stems with leaves, and the seedlings can be grown after maintenance and propagation(105 cell plug trays); there are also clusters of buds produced from the apical and axillary buds of germinated seeds, such as balsam balsam(cheap 4 inch plastic nursery pots suppliers).
Flowers usually germinate or produce adventitious buds by abdominal buds, and then carry out maintenance and proliferation culture by bud propagation(cheap 4 inch plastic nursery pots suppliers); Protocorm proliferation pathway can be used for some Orchidaceae and Liliaceae plants(288 cell plug tray). According to the characteristics of the above-mentioned propagation method, the most effective propagation method can be selected for propagation and cultivation.
Generally, when the shoot shoots the stem and grows to a certain height, the seedling is cut from the callus and transferred to the rooting medium(13cm plastic plant pots). The use of plant rapid propagation technology for the factory production of seedlings has become an emerging industry in many countries(32 cell plug tray). Only with the above conditions can we meet the requirements of industrialized and commercial production of asexual reproduction(cheap 4 inch plastic nursery pots suppliers).
The callus will be formed at the point where the stem is cut, and the root will be further induced to form a complete plant(200 cell seed trays). When the callus is transferred to the differentiation medium, the tissue is differentiated(cheap 4 inch plastic nursery pots suppliers). At present, there are not many plant species and varieties that can induce embryoid bodies, and their occurrence mechanism is unique to further exploration, and some still have certain genetic variations(50 deep cell plug trays).
The protocorm is transferred to a solid medium to extract leaves and roots to form a complete plant(seedling tray 104 holes). This method firstly differentiates the callus. They should be tested and tested before they can be used in large quantities in production(cheap 4 inch plastic nursery pots suppliers). There are three main ways of proliferation commonly used in the rapid propagation of flower waste, namely limb bud germination, induction of adventitious buds and embryogenesis.
This approach can quickly obtain one plant after another, which is relatively simple and effective for rapid propagation and preservation of certain flowers and tree species with excellent traits(162 cell seed starting trays). This method produces seedlings at one time, has stable genetic characteristics, simple cultivation process, wide application range, and easy survival after transplanting(200 cell plug trays). This is the most common way to propagate woody flowers(cheap 4 inch plastic nursery pots suppliers).
The time of Plant Regeneration from explants in vitro is short, that is, the reproductive cycle is short(cheap 4 inch plastic nursery pots suppliers). It has a high reproductive coefficient(72 cell plug trays). In addition, complete plants are developed from cells or calli through embryoid pathway through spherical embryo stage, heart-shaped embryo stage, torpedo embryo stage and cotyledon stage, and their development process is similar to that of zygotic embryo (fertilized egg).
The green protosphere base can be formed in 6 weeks(cheap 4 inch plastic nursery pots suppliers). Four conditions must be met for the rapid propagation of flowers in vitro for practical production(162 cell plug tray): stable genetic traits, stable chromosome number and stable physiological characteristics. The survival rate of test tube plantlets was high(128 cell plug trays). In order to improve the success rate of culture, The growth cones with 1 ~ 2 leaf primordia should be cut for meristem culture.
Because the scattered meristematic cell clusters near the surface of the gold wound tissue produce unidirectional polarity, the cell After dividing, the meristematic cells gradually increase and expand to the surface of the callus to become the shoot apical meristem(cheap 4 inch plastic nursery pots suppliers). Rapid propagation can be divided into four stages(50 cell plug trays): the establishment of sterile culture, bud proliferation, rooting and test tube seedling transplantation.
The selection of appropriate explants is very important for the success of rapid propagation, and the choice of explants first depends on the way of bud proliferation in the second stage(cheap 4 inch plastic nursery pots suppliers). When using the proliferation of axillary buds for rapid propagation, the selected explants should contain vegetative buds, and pay attention to the following problems at the same time(12.5 cm plant pots). The size of explants has a direct impact on the culture effect.
For different flower species, in order to obtain virus-free plants from plant culture materials without stock virus treatment, the smaller the explant, the better(cheap 4 inch plastic nursery pots suppliers). Generally, explants are slow or unresponsive to induction(128 cell seed trays). Most of the test-tube seedlings produced in this way have the problem of unstable fast-transmitting traits(105 cell plug flats). Since the 1960s, Huacai's clonal rapid propagation technology has been rapidly developed.
In this way, the position of explant also has a certain influence on the culture effect(cheap 4 inch plastic nursery pots suppliers). Generally, the explants in the lower part of the plant are easy to culture successfully, while the explants in the upper part are difficult to start and differentiate. The smaller the explant, the less virus or other pathogens, but it is difficult to survive(32 cell seedling tray); The larger the explant, the more virus it carries, the easier it is to pollute, but the better the culture.
If it is only general rapid propagation or when the raw materials have been identified to be free of virus, the influence of sampling season is also very important(cheap 4 inch plastic nursery pots suppliers). For example, Lily Scale explants are easy to form small bulbs in spring and autumn, while it is difficult to form small bulbs in summer and winter(98 cell seed tray). Then, most plants should take materials in the season at the beginning of their growth, at the end of growth or in dormancy.