Cytokinins (KT, Ba, zeatin, 2-ip) are dissolved with a small amount of in hydrochloric acid, then slowly add water to fix the volume, and pour into the bottle for storage(cheap plastic plant pots wholesale price slovenia). Now, taking MS medium as an example, the preparation method of mother liquor is introduced(plastic flower pots for sale). If it is distilled water, the water quality is often affected by the water source.
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The mother liquor concentration of cytokinin is better to be lower(4 inch plant pots). It is easy to dissolve at low concentration and no re knot occurs. Vitamins (VB, VB, VB: etc.), amino acids, niacin, etc. can be directly dissolved in water. In facility substrate cultivation and hydroponic cultivation, forced ventilation can be used to increase gas exchange(cheap plastic plant pots wholesale price slovenia). Soil acid-base environment, that is, the pH of the soil (expressed as the value of soil p).
Yeast extract is usually prepared temporarily when preparing culture medium(large plastic nursery pots). The prepared mother liquor shall be stored in a refrigerator at 2 ~ 4 ℃. It is best to add a small amount of potassium permanganate with non-ionic water that has not been re evaporated in advance, and then re steam the restaurant after placing it for a few days, so as to remove the organic substances in the water(cheap plastic plant pots wholesale price slovenia).
If precipitation occurs after storage, it can be reused after heating to dissolve it(cheap plastic plant pots wholesale price slovenia). The specific methods for preparing mother liquor of various additional components are as follows(black plastic planters): because coconut milk is rich in nutrition and is extremely resistant to storage, it can be filtered and sterilized by microporous membrane or stored in the refrigerator for standby after high temperature and high pressure sterilization.
The oxygen content in the soil air is generally only 10%~12%(black plastic nursery pots wholesale). Increase the gas exchange between the rhizosphere and the outside world. The prepared mother liquor shall be labeled respectively, indicating the mother liquor number, the number of letters to be prepared, the amount to be absorbed when preparing IL medium (NL), and finally the preparation date(20 gallon grow bags). The storage time should not be too long(cheap plastic plant pots wholesale price slovenia).
If mold and sediment are found, it should not be used again(large plastic plant pots for sale). In plant tissue culture, chemical pure CP (grade III) or analytical pure AR (grade II) drugs with higher grade should be used to avoid adverse effects of impurities in drugs on cultures(plastic growers pots suppliers). It is best to use it up within one month(cheap plastic plant pots wholesale price slovenia). When preparing the culture medium, all preparations shall be made in advance and carried out according to the preparation procedure.
Therefore, weigh the yeast powder according to the dosage, add water and heat it to boil for 30 minutes, then filter the residue and take the clear liquid(5 inch nursery pots). Coconut milk take the coconut milk from the coconut and remove the residue with several layers of cloth (or filter paper). Put the filtrate into an 80 ℃ water bath for 20 minutes(cheap plastic plant pots wholesale price slovenia). After cooling, filter the protein with filter paper, and keep the clear liquid for standby.
In particular, the tap water in big cities has poor water quality and often contains organic substances such as phenols(cheap plastic plant pots wholesale price slovenia). It is not always possible to evaporate them in Yanguan(nursery pot suppliers). In case of mildew and rain season, the sterilization time can be appropriately extended by 1 ~ 2 minutes. Generally add distilled water until the final volume of the medium is 1 liter(plastic hanging baskets for sale). Further increase of CO2 will have a toxic effect on plants.
Add about 750ml distilled water into the clean stainless steel pot, put the weighed specified amount of agar and barbiturate into the pot, heat and stir until the agar dissolves (there is no need to heat when preparing liquid culture medium)(nursery trays for plants). Add the stock mother liquor in the table in order, and add plant hormones and other special additional components as required according to the purpose of culture(cheap plastic plant pots wholesale price slovenia).
Generally speaking(injection molded nursery containers), pour the culture medium into the lower cup while it is hot, and distribute the culture medium into the sterilized culture container through the alligator clip on the rubber tube of the lower cup(cheap plastic plant pots wholesale price slovenia). The culture medium solution is about 14～14 of the ancient container. l3 is appropriate(plastic tree pots wholesale). Use a cotton stopper wrapped in gauze (previously sterilized) or other suitable stopper to seal the bottle tightly.
When aliquoting, be careful not to stick the medium solution on the bottle mouth or the inner wall near the bottle mouth, so as not to cause pollution in the future(8 inch plastic plant pots). The mother liquor calculated as the number of milligrams per milliliter is conducive to the preparation of various culture media(cheap plastic plant pots wholesale price slovenia). Since plant tissue culture requires aseptic conditions, the medium and the utensils and utensils used need to be sterilized.
Too low concentration of 2 and too high concentration of CO2 in the soil rhizosphere gas environment will inhibit the growth of crop roots(large plastic plant pots for trees). When distributing, always stir the medium in the lower cup to make the medium solution even, otherwise the degree of solidification of the medium in each bottle that is subdivided successively will be different(cheap plastic plant pots wholesale price slovenia). Take the preparation of 1 liter of MS medium as an example.
Put the culture container filled with the culture medium into the pressure cooker(plastic planters canada), the pressure gauge read 1.1kgcm during sterilization, and keep the temperature at 122°C for 20 minutes. After mixing well, adjust the p value of the medium with 0.1~IN NaOH and 0.1~IN H1 (measured with a precision pH test paper or acidity meter) to make the pH of the medium The value is 5.8(cheap plastic plant pots wholesale price slovenia). If it is acidic, the medium will not freeze(nursery bags suppliers).
In the case of soil compaction or stagnant water and poor air permeability, it can be reduced to less than 10%(15 gallon pots for sale). At this time, it will inhibit the respiration of plant roots and affect the physiological functions of plants. For example, when the concentration of O2 in the rhizosphere of cucumber is above 15%, it has little effect on the photosynthesis of the plant(cheap plastic plant pots wholesale price slovenia); when the concentration of CO2 is 1% to 3%, the growth is inhibited.
The indirect effect of beginners is to affect the effectiveness of nutrients(plastic plant pots ireland), which may reduce or even invalidate the effectiveness of nutrients(cheap plastic plant pots wholesale price slovenia). In the process of vegetable cultivation and management in the greenhouse, attention should be paid to increasing the application of organic fertilizer, timely cultivation, increasing gas exchange, and creating conditions to make the soil form a good aggregate structure(five gallon nursery pots).
However, in poorly aerated soil, the concentration of CO2 can often reach 10%~15% (or even 3000 mg/L), which is not conducive to the development of plant roots and seed germination(greenhouse nursery pots). Destroy the respiratory function of the root system, and even cause plants to suffocate and die. When weighing different chemicals, different spoons shall be used to avoid mixing drugs and affecting the accuracy of drug weighing(cheap plastic plant pots wholesale price slovenia).